• Aging Lens Epithelium and Ferroptosis Susceptibility

  2026-09-13

  Wei and colleagues show that aging lens epithelial cells become unusually vulnerable to ferroptosis, linking age-related redox imbalance, lipid peroxidation, iron accumulation, and impaired glutathione protection. Their combination of human and mouse models, glutathione perturbation, RSL3 and erastin exposure, and transcriptome analysis provides a mechanistic framework for studying ferroptosis in cataractogenesis.

• WEHI-539: A BCL-XL Inhibitor Workflow

  2026-09-12

  WEHI-539 is a selective BCL-XL inhibitor for separating BCL-XL dependence from broader apoptotic stress. This practical guide connects dose-response testing, mitochondrial apoptosis assays, cancer stem cell sensitization, and MCL-1-focused combination studies.

• 1-methyl Adenosine Assay Workflows

  2026-09-11

  Build reliable 1-methyl Adenosine workflows for cell perturbation, intracellular nucleoside profiling, and biomarker discovery. The practical strategy combines controlled dosing with stable isotope-diluted UHPLC–MS/MS, chromatographic isomer separation, and matrix cleanup.

• MK-571 Workflows for Inflammation Research

  2026-09-11

  MK-571 (L-660,711) helps separate cysteinyl leukotriene signaling from ABCC1/MRP1-linked drug transport in airway and macrophage assays. This practical guide covers assay setup, dosing logic, cross-model controls, and troubleshooting for bronchoconstriction, allergic inflammation, and chemotherapy-stress workflows.

• RSL3 Workflows for GPX4-Driven Ferroptosis

  2026-09-10

  RSL3 provides a fast, mechanistically focused way to test GPX4-dependent ferroptosis, from HCC pathway studies to RAS-driven cancer models. This guide combines practical handling, quantitative assay conditions, TEAD2-informed experimental design, and troubleshooting for more reproducible oxidative cell-death results.

• Schizophrenia Risk Pathways in Developing Neurons

  2026-09-10

  Liu et al. used cell-type-resolved human iPSC models to show that schizophrenia-associated loci converge on different developmental pathways in cortical interneurons and glutamatergic neurons. The study links interneuron risk loci to PKC hyperactivity and arborization defects, while glutamatergic-neuron loci affect ion transport and calcium currents, providing a framework for cell-specific mechanistic studies.

• Amiloride (MK-870) for Ion Transport Workflows

  2026-09-09

  Amiloride (MK-870) enables controlled perturbation of epithelial sodium transport, with practical value in sodium channel research, cystic fibrosis research, and hypertension research. Its negative result in a reovirus-entry study also makes it a useful specificity control when testing cellular endocytosis modulation rather than a presumed universal uptake inhibitor.

• SM-164: Reliable Apoptosis Assay Workflows

  2026-09-09

  This scenario-based guide explains how SM-164 (SKU A8815), a bivalent Smac mimetic, can support interpretable viability, proliferation, and apoptosis experiments. It covers mechanism, solvent compatibility, handling, orthogonal readouts, and practical criteria for selecting a reliable research reagent.

• Sinapine Targets PLCβ3 EF Hands in Cardiovascular Disease

  2026-09-08

  The reference study identifies the PLCβ3 EF-hand domain, particularly Asn-260, as a druggable site for disrupting the Gαq–PLCβ3 interaction in RAAS-driven cardiovascular disease. By combining chemical-probe target identification, biochemical validation, and aldosteronism and hypertension models, the authors position sinapine as a more pathway-focused alternative to broad Gαq or PLC inhibition.

• Primidone (Mysoline) for TRPM3 and RIPK1 Research

  2026-09-08

  Primidone, also known as Mysoline, gives researchers a practical bridge between TRPM3 channel pharmacology, RIPK1 signaling, and translational disease models. This guide covers assay setup, concentration selection, animal-study context, CYP19 counter-screening, and troubleshooting for reproducible workflows.

• Silymarin: Silybin A Workflow for Liver Research

  2026-09-07

  Build reproducible Silybin A experiments around solubility control, orthogonal viability and motility assays, and pathway-aware validation. This workflow distinguishes purified Silybin A from Silymarin mixtures while adapting a hepatocellular carcinoma metastasis assay framework without overstating evidence.

• O6-Benzylguanine MGMT Inhibitor Workflows

  2026-09-07

  Use O6-Benzylguanine to separate MGMT-dependent DNA repair from broader transcriptional resistance mechanisms and to design stronger alkylator-sensitization experiments. This practical workflow connects stock preparation, combination assays, DNA-damage readouts, and the AP-2α–MGMT findings in recurrent glioblastoma research.

• Erastin Workflow for Ferroptosis Research

  2026-09-05

  Build reproducible ferroptosis assays with Erastin by controlling DMSO handling, cell density, exposure time, and orthogonal oxidative-stress readouts. The workflow also connects RAS/BRAF genotype, system Xc⁻ activity, and PERK–SLC7A11 biology to practical cancer biology research decisions.

• GSK3 Inhibition Controls M. tuberculosis Infection

  2026-09-04

  The 2024 iScience study identifies glycogen synthase kinase 3 as a host pathway that supports intracellular Mycobacterium tuberculosis growth in macrophages. Through phenotypic screening, genetic validation, and phospho-proteomic analysis, the authors show that GSK3 inhibition promotes an antimicrobial macrophage state and provides a mechanistic basis for host-directed tuberculosis therapy.

• TH287 MTH1 Inhibitor for Cancer Radiosensitization

  2026-09-04

  TH287 converts oxidized nucleotide stress into a measurable DNA-damage phenotype and offers a practical route to studying cancer cell selective cytotoxicity. In castration-resistant prostate cancer models, schedule-dependent combination with ionizing radiation provides a focused workflow for testing radiosensitization, apoptosis, and cell-cycle disruption.

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